Figure 3: Images of HUVECs treated with amine modified polystyrene nanoparticles.
From: Real-time and label-free monitoring of nanoparticle cellular uptake using capacitance-based assays
(a) Real-time capacitance results and time lapse confocal images of HUVECs during the internalization of the NH3+-PNPs (green) into the cell membrane (light yellow area). (b) Confocal microscopy of amine-modified PNPs uptake by HUVECs. Confocal cross-section and z-stack images of HUVECs show internalization of NH3+-PNPs 109, 108 and 107 PNPs/well. Scale bars, 10 μm and stack depth, 8 μm. (c) HUVECs’ uptake of both NH3+-PNPs (green) and LysoTracker® Red (left); the same, pretreated with cytochalasin D (middle); and the same, pretreated with chlorpromazine (right). The yellow regions indicate co-localization of NH3+-PNPs with LysoTracker in the superimposed images. Scale bars, 10 μm.
