Figure 5: Time- and frequency-dependent capacitance values in siCD44-lipofectamine 2000 treated cells.
From: Real-time and label-free monitoring of nanoparticle cellular uptake using capacitance-based assays
(a) Time-dependent normalized capacitance values for HUVECs treated with lipofectamine 2000 alone and siCD44-lipofectamine 2000 at 24 hour (gray arrow). Time-dependent estimates of α (b) and β (c) from real-time capacitance measurements using a capacitance sensor array (n = 5). (d) The relative gene expression level of CD44 measured by real-time PCR (n = 3). *vs control, P < 0.001. (e) Confocal cross-section images of HUVECs showing internalization of siCD44-lipofectamine 2000 complex with FITC (green). (f) HUVECs uptakes of siCD44-lipofectamine 2000 complex with FITC (green) and then treated with LysoTracker® Red. The yellow regions indicate co-localization of siCD44-lipofectamine 2000 complex with LysoTracker in the superimposed images. Scale bars, 10 μm. Full data are presented in Supplementary Fig. 10.
