Figure 2: Evolution of CCR7 or CD2 RNA aptamers via live cell-based HT-SELEX. | Scientific Reports

Figure 2: Evolution of CCR7 or CD2 RNA aptamers via live cell-based HT-SELEX.

From: High throughput sequencing analysis of RNA libraries reveals the influences of initial library and PCR methods on SELEX efficiency

Figure 2

(a,b) The molecular enrichment at each round of HeLa-CCR7 cell-SELEX (a) and HeLa-CD2 cell-SELEX (b). The molecular enrichment at each round was calculated by the formula: total reads of top 1000 sequences in round X/unselected library (initial library). (c,d) The frequency of each group at each selection round of HeLa-CCR7 cell-SELEX (c) and HeLa-CD2 cell-SELEX (d). After alignment of the top 50 sequences, several groups of aptamers were identified. The percent frequency of each group at each selection round was calculated by the formula: the reads of each group/the total reads of top 1000 unique sequences. (e) The fate of biased sequence from initial RNA libraries during SELEXs. (f) Cell-type specific binding of selected RNA aptamers. Cy3-labeled RNAs were tested for binding to H9 (CCR7highCD2low) and Jurkat (CCR7lowCD2high). PE-CF594-conjugated anti-human CCR7 antibody or PE-conjugated anti-human CD2 antibody was used to stain cellular surface CCR7 or CD2, respectively. Data represent the average of triplicate measurements. Error bars represent SD.

Back to article page