Table 1 Bioinformatics analysis of high throughput sequencing data from the initial RNA libraries.

From: High throughput sequencing analysis of RNA libraries reveals the influences of initial library and PCR methods on SELEX efficiency

A-derived initial RNA pool

A

C

T

G

Solution PCR

25.48%

23.18%

24.72%

26.63%

ddPCR

23.86%

24.67%

22.75%

28.72%

B-derived initial RNA pool

A

C

T

G

Solution PCR

22.50%

22.60%

28.10%

26.80%

ddPCR

19.90%

24.40%

24.50%

31.20%

  1. ssDNA libraries purchased from different companies (A and B) were used to generate dsDNA libraries by either solution PCR or ddPCR amplification. dsDNA libraries were subsequently converted into RNA libraries via in vitro transcription. The nucleotide compositions (A, C, T, and G) in the 30-nt random region were identified.
Back to article page