Table 11 The nucleotide compositions (A, C, T and G) in the random region were identified.

From: High throughput sequencing analysis of RNA libraries reveals the influences of initial library and PCR methods on SELEX efficiency

RNA library

Solution PCR-driven SELEX

ddPCR-driven SELEX

A

C

T

G

purine

pyrimidine

A

C

T

G

purine

pyrimidine

Initial library

22.50%

22.60%

28.10%

26.80%

49.30%

50.70%

19.90%

24.40%

24.50%

31.20%

51.10%

48.90%

Round 1

21.00%

23.30%

29.00%

26.80%

47.80%

52.30%

19.20%

24.40%

24.10%

32.20%

51.40%

48.50%

Round 3

18.40%

24.30%

32.90%

24.30%

42.70%

57.20%

17.90%

25.00%

25.20%

31.90%

49.80%

50.20%

Round 5

15.20%

24.30%

37.40%

23.10%

38.30%

61.70%

16.00%

24.50%

26.20%

33.30%

49.30%

50.70%

Round 6

14.40%

23.90%

39.40%

22.30%

36.70%

63.30%

15.80%

25.00%

26.20%

32.90%

48.70%

51.20%

Round 7

14.00%

23.00%

41.10%

22.00%

36.00%

64.10%

15.50%

24.80%

26.20%

34.00%

49.50%

51.00%

  1. The initial RNA libraries and several RNA libraries from selection rounds 1, 3, 5, 6 and 7 in solution PCR-driven SELEX or ddPCR-driven SELEX were analyzed by high throughput sequencing.
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