Figure 2
From: MBD3 expression and DNA binding patterns are altered in a rat model of temporal lobe epilepsy
Expression pattern of Mbd3 mRNA in normal and stimulated rat brain.
Cellular localization of Mbd3 transcript in the piriform cortex in sham-operated rats (A) and amygdala-stimulated rats 14 days after status epilepticus (B). Representative confocal microscopy images show Mbd3 mRNA (green), detected by in situ hybridization and neuronal cells, oligodendrocytes and astrocytes (red), detected by immunohistochemistry using NeuN, Olig2 and GFAP antibodies, respectively. Cell nuclei were counterstained with DAPI (blue). Typical examples of triple-labeled cells are indicated by arrows. Olig2- and GFAP-positive cells that lack Mbd3 mRNA are marked with asterisks. DAPI. 4,6-diamidino-2-phenylindole; GFAP, glial fibrillary acidic protein; NeuN, neuron-specific nuclear protein; Olig2, oligodendrocyte lineage transcription factor 2. Scale bar = 10 μm.
