Figure 6
From: MBD3 expression and DNA binding patterns are altered in a rat model of temporal lobe epilepsy
Composition of MBD3-containing complex and cellular localization of NuRD subunits in the brain.
(A) Representative immunoblotting with antibodies directed against MTA1, MTA2, HDAC1, HDAC2 and GATAD2B proteins in lysates of temporal lobe structures subjected to immunoprecipitation with MBD3 antibody. (B) Cellular localization of MTA2 and HDAC2 proteins in the piriform cortex in sham-operated animals. (C) Cellular localization of MTA2 and HDAC2 proteins in the piriform cortex in epileptic rats 14 days after amygdala stimulation. (B,C) Representative confocal microscopy images show MTA2 and HDAC2 proteins (green) and neuronal cells, astrocytes and microglia (red), detected by immunohistochemistry using NeuN, GFAP and CD11b antibodies, respectively. Cell nuclei were counterstained with DAPI (blue). Typical examples of triple-labeled cells are indicated by arrows. Examples of microglial cells that do not express MTA2 and HDAC2 proteins are indicated by arrowheads. CD11b, cluster of differentiation molecule 11B, integrin αM chain; DAPI, 4,6-diamidino-2-phenylindole; GATAD2B, GATA zinc finger domain containing 2B; GFAP, glial fibrillary acidic protein; HDAC2, histone deacetylase 2; MBD3, methyl-CpG binding domain protein 3; MTA1, metastasis associated 1 family, member 1; MTA2, metastasis associated 1 family, member 2; NeuN, neuron-specific nuclear protein;. Scale bars = 10 μm. Full length scans of Western Blots used for images cropped to the Fig. 6 are presented in Supplementary Figure 1.
