Table 1 Proteins coprecipitated with MBD3 in the immunoprecipitation assay with the antibody directed against MBD3 followed by mass spectrometry.
From: MBD3 expression and DNA binding patterns are altered in a rat model of temporal lobe epilepsy
Protein name | Selected protein functions | Peptide # | |
|---|---|---|---|
Anty-IgG | anty-MBD3 | ||
PPP2CB, Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform | modulates the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase and MAP-2 kinase | 0 | 5 |
APAF1, Apoptotic protease-activating factor 1 | mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis | 0 | 5 |
HDAC2, Histone deacetylase 2 | deacetylates lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) | 0 | 3 |
MBD3, Methyl-CpG binding domain protein 3 | acts as transcriptional repressor and plays a role in gene silencing; binds to DNA with a preference for sites containing methylated CpG dinucleotides; recruits histone deacetylases and DNA methyltransferases | 0 | 2 |
ILF2, Interleukin enhancer-binding factor 2 | transcription factor regulating transcription of the IL2 gene | 0 | 2 |
NEFM, Neurofilament medium polypeptide | involved in the maintenance of neuronal caliber | 0 | 2 |
GATAD2B, Transcriptional repressor p66-beta | Enhances MBD2-mediated repression; targets MBD3 to discrete loci in the nucleus | 0 | 1 |
RBBP4, Histone-binding protein RBBP4 | core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA; component of several complexes which regulate chromatin metabolism. | 0 | 1 |
MTA1/2, Metastasis-associated protein 1/2 | transcriptional coregulator which can act as both a transcriptional corepressor and coactivator. | 0 | 1 |
SRSF1, Serine/arginine-rich splicing factor 1 | can either activate or repress splicing, depending on its phosphorylation state and its interaction partners | 0 | 1 |
AMPD3, Adenosine monophosphate deaminase 3 | plays a critical role in energy metabolism. | 0 | 1 |
PPP1R9A, Neurabin-1 | binds to actin filaments; may be involved in neurite formation | 0 | 1 |
AAK1, AP2-associated protein kinase 1 | Regulates clathrin-mediated | 0 | 1 |
SYT7, Synaptotagmin-7 | involved in Ca2+-dependent exocytosis of secretory vesicles | 0 | 1 |
