Figure 7
Cartoon showing MelB opening.
According to the model proposed by Fowler et al.5 for oligopeptide transporters, the pairs of helices TM4–TM5 (orange) and TM10–TM11 (green) define the cytosolic gate, whereas the pairs of helices TM1–TM2 (mauve) and TM7–TM8 (blue) define the periplasmic gate; gates indicated by a dashed red ellipsoid in our MelB opening. The opening of MelB towards the periplasmic side (dashed red arrowed lines) barely affects the cytosolic gate, dividing the protein in two halves at the level of the TM2–TM11 knob (red-line yellow dot).
