Figure 2 | Scientific Reports

Figure 2

From: Mislocalisation of BEST1 in iPSC-derived retinal pigment epithelial cells from a family with autosomal dominant vitreoretinochoroidopathy (ADVIRC)

Figure 2

Reprogramming and characterisation of ADVIRC patient-derived iPSCs.

(A) Growth of fibroblast cells from ADVIRC patient skin explant. Scale Bar 200 μm (B) Induced pluripotent stem cell colony reprogrammed from ADVIRC patient. Scale bar 1000 μm (C) Immunocytochemistry for stem cell markers confirming pluripotency of ADVIRC patient-derived iPSC. Scale bar for NANOG and TRA-1-60 100 μm and OCT4 and DAPI 500 μm. (D) Taqman® hPSC Scorecard ™ assay results comparing undifferentiated human embryonic stem cells (HESC), undifferentiated control iPSCs (Control iPSC), undifferentiated patient iPSC (ADVIRC iPSC) and differentiated patient iPSC (ADVIRC EB). Scatter plots are shown in the upper right of the matrix and corresponding coefficient of determination (R2) in the lower left. (E) Teratoma assay of H&E stained sections taken from ADVIRC patient derived iPSC 8 weeks following injection into NOD-SCID mice displaying tissue from the three germ cell lineages. (F) Immunocytochemistry staining of ADVIRC-iPSC derived teratoma sections showing differentiation of pigmented epithelial cells that express the pre-melanosomal protein, Pmel17. Scale bar 100 μm.

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