Figure 1
From: Preferential uptake of antioxidant carbon nanoparticles by T lymphocytes for immunomodulation
PEG-HCCs are preferentially internalized by T lymphocytes.
(a) PEG-HCC levels in rat serum determined by anti-PEG ELISA after a single subcutaneous injection of 2 mg/kg body weight PEG-HCCs (left) (n = 5 rats/time-point). Data fit to a single exponential decay to calculate circulating half-life of 27 h (right). (b) FCM quantification of immune cell uptake of PEG-HCCs from spleen (left, n = 9 rats) and lymph nodes (right, n = 6 rats), collected 24 h after subcutaneous injection. The increased PEG signal after cell permeabilization is indicative of nanoparticle internalization; analyzed with two-way ANOVA. (c) Quantification of FCM data showing preferential uptake of PEG-HCCs by T cells over other splenic immune cells ex vivo (n = 3 spleens, two-way ANOVA). Mean ± s.e.m. **P < 0.01, ***P < 0.001.
