Figure 3

PEG-HCCs internalization is improved in activated T cells but is not biased towards major T cell subsets.

(a) PEG-HCC internalization in naïve (CD3⁺CD62L⁺) and memory (CD3⁺CD62L⁻) rat splenic T cells, analyzed by FCM (n = 3 splenic preparations). (b) PEG-HCC internalization in helper (CD3⁺CD4⁺, left) and cytotoxic (CD3⁺CD8⁺, right) rat splenic T cells, analyzed by FCM (n = 3 splenic preparations). A separate group of rat splenocytes cells were also stimulated with the mitogen, concanavalin A (1 μg/mL). (c) PEG-HCC internalization in the δγ T cell subset (CD3⁺δγ⁺) as compared to non-δγ T cells (CD3⁺δγ⁻) from rat splenocytes, analyzed by FCM (n = 3 splenic preparations). (d) PEG-HCC internalization in primary CD3⁺CD4⁺, T_H1-polarized effector memory rat T cells, analyzed by FCM (n = 3 splenic preparations). Mean ± s.e.m. *P < 0.05, **P < 0.01, ***P < 0.001.