Figure 1: Unstructured peptide-insertion screening.

(A) TRPV1 residues bracketing the insertion sites are highlighted in green (functional) and red (non-functional). The linker between the ankyrin-like repeat domain and S1 is shown in blue. (B) A schematic diagram showing the membrane topology of a TRPV1 subunit. Insertion sites that yield functional and non-functional mutants are marked by green and red triangles, respectively. (C – F) Zoom-in view of the channel structures containing peptide insertions. Insertions are named in the format of Xnnn_maa, in which X represents the single-letter amino acid preceding the insertion, nnn represents the residue number, m represents the number of amino acids in the inserted peptide.