Figure 2: Confocal imaging of cellular distribution of expressed channel proteins.

(A) Images of a HEK293 cell expressing the wild-type TRPV1_eYFP (left three panels) reveal substantial overlap between eYFP (yellow) and the plasma membrane dye di-8-ANEPPS (red). Images of cells expressing a mutant channel (Q561_8aa) are shown in the right panel. The fluorescence intensity profile from a line scan (indicated by dash line) is shown on the right side. (B) Representative images of mutant-expressing cells and the fluorescence intensity profile.