Figure 5: Peptide insertion before the S2-S3 linker does not disrupt capsaicin activation. | Scientific Reports

Figure 5: Peptide insertion before the S2-S3 linker does not disrupt capsaicin activation.

From: Exploring functional roles of TRPV1 intracellular domains with unstructured peptide-insertion screening

Figure 5

(A) Structural models of the S2-S3 linker (orange) superimposed on the electron density map of TRPV1 in the apo state (EMD-5778). The position of L504 is highlighted in green; the side-chain of Y512 and S513 are shown in ball-and-stick format (purple). (B,C) Representative current trace of WT and L504_8aa mutant channel in response to increasing concentrations of capsaicin, respectively. (D) The concentration-response relationship of L504_8aa is fitted to a Hill function with EC50 of 0.19 ± 0.08 μM and slope factor of 0.6 ± 0.05 (n = 3). The wild-type TRPV1 curve (black dashed curve) is shown as comparison.

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