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Figure 1

From: Construction of a Corneal Stromal Equivalent with SMILE-Derived Lenticules and Fibrin Glue

Figure 1

Serial construction of the corneal anterior lamellar.

First, the lenticule is extracted from the human cornea using the VisuMax Femtosecond Laser System. Then, 2 lenticules derived from the same donor are adhered to each other by fibrin glue and soaked in culture medium for 24 h. Finally, human limbal epithelial cells are seeded on top of the constructed corneal stromal equivalent. The air-liquid culture technique is used to induce epithelial stratification.

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