Figure 1: Chemical structures of DON and its sulfates and LC-MS/MS identification of DON-3-sulfate. | Scientific Reports

Figure 1: Chemical structures of DON and its sulfates and LC-MS/MS identification of DON-3-sulfate.

From: Identification of a novel human deoxynivalenol metabolite enhancing proliferation of intestinal and urinary bladder cells

Figure 1

Structures (a) of deoxynivalenol (1), DON-3-sulfate (2) and DON-15-sulfate (3) as well as SRM-chromatograms and MS/MS spectra of authentic reference standards (b) and a naturally contaminated urine sample (c). The reference (b) contains a mixture of DON-3-sulfate and DON-15-sulfate, whereas in the naturally contaminated urine sample (c) only DON-3-sulfate is present. Based on a comparison of the retention time and the observed fragments with the standard substance the isomer in the urine sample was identified as DON-3-sulfate. MS/MS scans were recorded at a collision energy of −20 eV.

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