Figure 4 | Scientific Reports

Figure 4

From: Identification of hepta-histidine as a candidate drug for Huntington’s disease by in silico-in vitro- in vivo-integrated screens of chemical libraries

Figure 4

Expected common structure of candidate chemicals.

(a) The structures of six chemicals that were effective for the Drosophila HD model are merged to extract the common features of the ternary structure. F1 is a donor/acceptor for hydrogen bonds and F2-4 are acceptors for hydrogen bonds. The positions for the hydrogen bond are common. The covalent bonds are colored red if the position is shared among five chemicals and are colored blue if the position is shared among the six chemicals. (b) The incorporation of 7H at the dip in the N-terminal region of Ku70 was visualized using Discovery Studio. (c) CD spectra of the three peptides, 7H, AngIII and LH-RH 4–10 partial peptide, are shown. In each panel, solid line, dashed line and dotted line stand for 0, 10 and 20% HFIP, respectively. (d) Co-IP of Ku70 and mutant Htt with cerebral cortex samples from R6/2 treated with 7H. The results obviously show decreased interaction between Ku70 and mutant Htt after treatment of 7H.

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