Table 1 Primers for qRT-PCR.
From: Porcine milk-derived exosomes promote proliferation of intestinal epithelial cells
Gene name | Primer | sequence (5′ to 3′) |
|---|---|---|
IGF-1R (mouse) | IGF-1R -F | GGCAAGTATGCGTGAAAGAATC |
IGF-1R -R | CTAAAGGTCGGAGGAATGAGG | |
PCNA (mouse, pig) | PCNA -F | AGATGCCGTCGGGTGAAT |
PCNA -R | TCTCTATGGTTACCGCCTCCT | |
p53 (mouse, pig) | P53-F | CATTGTCAGGCTTATGGAAACTAC |
P53-R | ACACTCGGAGGGCTTCACTT | |
Cdx2 (mouse, pig) | Cdx2-F | ACCGCAGAGCCAAGGAGA |
Cdx2-R | AGGAGGTCACAGGAGTCAAGG | |
β-actin (mouse, pig) | Beta-actin -F | TGCTGTCCCTGTATGCCTCT |
Beta-actin | CTTTGATGTCACGCACGATTT | |
IGF-1R (pig) | IGF-1R -F | GAACCGCATCATCATCACC |
IGF-1R -R | CATCCTGCCCATCATACTCC | |
FAS(pig) | FAS-F | GATTTACCTGTATCGCTGGACC |
FAS-R | AGCAGAATGGACCCTCACG | |
SERPINE(pig) | SERPINE-F | CTACTTCTTCAGGCTGTTCCG |
SERPINE-R | AGGCAGTGGTGAGTGCTTTT | |
miR-7314 | ATGCGGAACCTGCGGATAC | |
miR-1343 | TATTATCTCCTGGGGCCCGC | |
miR-769-3P | CTGGGATCTCTGGGGTCTTGGTT | |
miR-181a | AACATTCAACGCTGTCGGTGAGTT | |
miR-128 | TCACAGTGAACCGGTCTCTTT | |
miR-2320 | TGGCACAGGGTCCAGCTGTCGG |
