Figure 8

T cell exhaustion in tolerant recipients after MyD88, TRIF and mTOR siRNA treatment.

Mice were treated and transplanted with allografts as described in Fig. 5. The splenic T cells were isolated from different experimental groups as indicated. RNA was extracted and transcripts of PD-1, TIM-3 were determined using real time RT-PCR. Data shown are mean ± SEM (n = 6, *P<0.01 vs control groups).