Table 2 Primers used in 5′RACE.
From: Humanization of high-affinity antibodies targeting glypican-3 in hepatocellular carcinoma
Primer’s name | Sequence | Usage |
|---|---|---|
MG1-Hinge_708-683R | ACCACAATCCCTGGGCACAATTTTCT | RT for IgG1 heavy chain |
MG1-PCR_438-409R | AGGGGCCAGTGGATAGACAGATGGGGGTGT | 1st PCR for IgG1 heavy chain |
MVH2R | ATAGACAGATGGGGGTGTCGTTTTGGC | 2nd PCR for IgG1 heavy chain |
MK-Edge_711-685R | CTCATTCTTGTTGAAGCTCTTGACAAT | RT for Kappa chain |
MK-PCR_435-403R | GGATGGTGGGAAGATGGATACAGTTGGTGCAGC | 1st PCR for Kappa chain |
Adaptor + G | GGCCACGCGTCGACTAGTACGGGGGGGGGG | 1st PCR |
Adaptor | GGCCACGCGTCGACTAGTAC | 2nd PCR |
