Figure 3: Ezh2 deletion promotes derepression of Six1 and photoreceptor-related genes.
From: Postnatal onset of retinal degeneration by loss of embryonic Ezh2 repression of Six1
(a) Heat map of clustered microarray data from P0 retina showed 132 genes changed >1.5 fold between control and KO mice. (b) GO analysis of functional categories identified a high number of genes that are directly involved in photoreceptor development, differentiation and photosensitivity. (c,d) qPCR results from P0 WT and KO retina showed the relative expression of retinal progenitor cell (RPC) and neuron-related genes in KO mice at P0. RGCs, retinal ganglion cells; MC, Müller cells. Note the significant elevation of Six1, Eya2 and rod photoreceptor-related gene expression, including Nr2e3, Recoverin (Rcvrn), Nrl and Rhodopsin (Rho), in KO mice. Bars represent the mean ± S.D. of at least six biological replicates. NS indicates no significant difference; *indicates P < 0.05; **indicates P < 0.01; ***indicates P < 0.001.
