Figure 1

IL-22 mRNA expression is induced in D1 cells infected with bacteria.
D1 cells (4,5 × 105 cells/ml) were infected with (a) L. innocua (MOI 1:1000); (b) L. monocytogenes (MOI 1:20); (c) L. paracasei (MOI 1:1000); (d) L. lactis (1:1000). IL-22 mRNA was assessed by qRT-PCR at 4 h, 8 h and 24 h post infection. Target mRNA was normalized to 18S and expressed as fold stimulation over control (0 h, non- treated cells). t.p.i.: time post infection. Each measurement was performed in duplicate. The data represent the mean values of two independent experiments (±SD) for (a,b) and three experiments (±SD) for (c,d).