Figure 10
CD11c+ cells release IL-22 both in vitro and in vivo after stimulation with LPS.
(a) A group of mice (N = 5) were treated i.v. with 50 ug of LPS. After 3 h, the spleens were removed and CD11c+ cells were purified by magnetic beads isolation and cultured for 20 h. IL-22 was measured by ELISA. (b) Ex vivo CD11c+ cells were purified from the spleens of a group of mice (N = 4). CD11c+ cells were then stimulated with LPSr (5 ug/ml), CpG (5 ug/ml) and zymosan (10 ug/ml). After 20 h, supernatants were collected and tested for IL-22 production by ELISA. (c) DX5+ cells from RAG2 KO mice (N = 3) were purified by magnetic beads isolation. DX5+ and DX5- negative fraction were in vitro stimulated with LPS (5 ug/ml) and rmIL23. After 20 h, supernatants were collected and tested for IL-22 production by ELISA. (d) CD11c+ cells were pretreated for 30 min with JNK inhibitor SP600125 (20 uM) and then stimulated with LPS (5 ug/ml). After 20 h, supernatants were collected and tested for IL-22 production by ELISA. The data represent the mean values of at least three independent experiments (±SD). Student’s T test statistical significance is shown (**p < 0.01).
