Figure 7

Determination of the function of Bcsec14 and Bcsec31.
(A) The colony phenotype of wild-type, ∆Bcsec14, and ∆Bcsec31 strains two weeks after inoculation on PDA plates. (B) Radial growth rate on PDA plates. Data represent the mean ± S.D. of three biological replicates. (C) Quantitation of conidial production. Data represent the mean ± S.D. of three biological replicates. (D) Virulence of wild-type, ∆Bcsec14, and ∆Bcsec31 strains on apple fruits. Images are of lesions 4 days post inoculation (dpi). (E) Diameters of lesions induced by the wild-type, ∆Bcsec14, and ∆Bcsec31strains at 3 and dpi. Data presented represent the mean ± S.D. (n = 10). (F) Virulence of wild-type, ∆Bcsec14, and ∆Bcsec31 strains on detached tomato leaves. (G) Quantitation of lesion diameter of tomato leaves. Data presented represent the mean ± S.D. of three biological replicates. (H) SDS-PAGE of the secreted proteins obtained from the wild-type, ∆Bcsec14, and ∆Bcsec31 strains. Five μg of proteins from the samples were loaded into each lane. Molecular weight markers are visualized in the far left lane.