Table 1 Hits with the highest scores (SEQUEST) in mass spectrometry analyses of proteins from pull-down assay with HB-GAM and BSA magnetic beads from hippocampal neuronal cultures.

From: HB-GAM (pleiotrophin) reverses inhibition of neural regeneration by the CNS extracellular matrix

Accession

Description

Coverage BSA

#Peptides BSA

Score HBGAM_1

#Peptides HBGAM_1

Score HBGAM_2

#Peptides HBGAM_2

P63090

Pleiotrophin OS = Rattus norvegicus GN = Ptn PE = 1 SV = 1 - [PTN_RAT]

  

60.48

7

57.62

8

P11442

Clathrin heavy chain 1 OS = Rattus norvegicus GN = Cltc PE = 1 SV = 3 - [CLH_RAT]

0

 

42.81

10

50.11

15

P50137

Transketolase OS = Rattus norvegicus GN = Tkt PE = 1 SV = 1 - [TKT_RAT]

0

 

24.04

7

27.27

4

Q9EPH8

Polyadenylate-binding protein 1 OS = Rattus norvegicus GN = Pabpc1 PE = 2 SV = 1 - [PABP1_RAT]

0

 

17.45

5

23.63

6

P85845

Fascin OS = Rattus norvegicus GN = Fscn1 PE = 1 SV = 2 - [FSCN1_RAT]

0

 

15.33

3

13.67

3

P09330

Ribose-phosphate pyrophosphokinase 2 OS = Rattus norvegicus GN = Prps2 PE = 1 SV = 3 - [PRPS2_RAT]

  

13.82

3

10.67

2

Q4FZT9

26S proteasome non-ATPase regulatory subunit 2 OS = Rattus norvegicus GN = Psmd2 PE = 2 SV = 1 - [PSMD2_RAT]

0

 

13.44

2

3.14

1

P51653

Glypican-2 OS = Rattus norvegicus GN = Gpc2 PE = 1 SV = 1 - [GPC2_RAT]

  

12.36

3

9.39

3

Q9JMJ4

Pre-mRNA-processing factor 19 OS = Rattus norvegicus GN = Prpf19 PE = 2 SV = 2 - [PRP19_RAT]

0

 

12.14

3

20.66

7

Q63610

Tropomyosin alpha-3 chain OS = Rattus norvegicus GN = Tpm3 PE = 1 SV = 2 - [TPM3_RAT]

0

 

11.69

5

12.79

5

O88761

26S proteasome non-ATPase regulatory subunit 1 OS = Rattus norvegicus GN = Psmd1 PE = 2 SV = 1 - [PSMD1_RAT]

0

 

10.28

2

10.48

3

P61765

Syntaxin-binding protein 1 OS = Rattus norvegicus GN = Stxbp1 PE = 1 SV = 1 - [STXB1_RAT]

  

9.83

3

7.6

3

P18484

AP-2 complex subunit alpha-2 OS = Rattus norvegicus GN = Ap2a2 PE = 1 SV = 3 - [AP2A2_RAT]

0

 

9.33

3

14.58

4

P62250

40S ribosomal protein S16 OS = Rattus norvegicus GN = Rps16 PE = 1 SV = 2 - [RS16_RAT]

0

 

9.24

4

12.95

4

P18422

Proteasome subunit alpha type-3 OS = Rattus norvegicus GN = Psma3 PE = 1 SV = 3 - [PSA3_RAT]

  

9.21

1

6.99

1

P62832

60S ribosomal protein L23 OS = Rattus norvegicus GN = Rpl23 PE = 2 SV = 1 - [RL23_RAT]

0

 

9.11

2

15.01

3

P04897

Guanine nucleotide-binding protein G(i) subunit alpha-2 OS = Rattus norvegicus GN = Gnai2 PE = 1 SV = 3 - [GNAI2_RAT]

0

 

8.94

3

8.13

3

“P40329

”“Arginine–tRNA ligase cytoplasmic OS = Rattus norvegicus GN = Rars PE = 1 SV = 2 - [SYRC_RAT]”“

   

8.7

3

5.28

“P62703

”“40S ribosomal protein S4 X isoform OS = Rattus norvegicus GN = Rps4x PE = 2 SV = 2 - [RS4X_RAT]”“

   

7.97

3

15.66

P62716

Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform OS = Rattus norvegicus GN = Ppp2cb PE = 1 SV = 1 - [PP2AB_RAT]

0

 

7.97

2

6.97

3

  1. The table shows the 20 highest scoring hits (out of 334) in two independent samples from hippocampal primary neurons (E17-18) found by pull-down assay with a cross-linking reagent (DTSSP) and magnetic beads covalently linked to HB-GAM (HBGAM_1 and HBGAM_2) and BSA.