Table 1 Oligonucleotides used for qRT-PCR.
Gene | Name | Sequence (5′-3′) |
|---|---|---|
YGK3 | YGK3-F | TGTACGTCAGGGAAGGGAAA |
YGK3-R | GCATAAGGTCCTAGCCATTCAAT | |
DUR1,2 | DUR1,2-F | ATGTATCTGGTGGTTCCT |
DUR1,2-R | GCCAATCAGGTTGTTCAA | |
AVT4 | AVT4-F | GCTGGAGGCGACATAACG |
AVT4-R | AACCACTTGCTGATGACAATTTAG | |
ARG1 | ARG1-F | TTCTTACGAGGCAGGTATCTTG |
ARG1-R | AAGTCTTGTTGTCGGTGTAGG | |
CAR1 | CAR1-F | ACGGAATTAGAGCCCTCAATG |
CAR1-R | GGAATCTGTTCGCCTGGAC | |
DAL1 | DAL1-F | AGACGGAGCCACCTACTT |
DAL1-R | CACTACTGAGCCTATAACACCTTC | |
GAP1 | GAP1-F | GAAGCACCACTTGAAGAATAG |
GAP1-R | CCAGAGCCATAACCATAGC | |
CAN1 | CAN1-F | ATGGAGGATGGCATAGGTGATGA |
CAN1-R | GCGTTGGTCAGAGGTGTGGATA | |
BAT2 | BAT2-F | TCGTCCAGATATGAATATG |
BAT2-R | CAGGAACTAAGCACTTAT | |
YJR011C | YJR011C-F | CGCTTATTATTGGATAGTTGTGAGT |
YJR011C-R | CGACATTTAATCTGTTTAATTCCTGG | |
PEP4 | PEP4-F | CCATACGATTACACGCTTGAAGT |
PEP4-R | CGCAAGAAGGCATCACCAA | |
UGA4 | UGA4-F | ATCTGTGATGGGTGGTGG |
UGA4-R | AGCGGTAGGAATGGAACTTG | |
TMC1 | TMC1-F | CCACGCTAAGATAAAGAAATCAGA |
TMC1-R | CCGCACTTGAACAGGTATC | |
RTG3 | RTG3-F | GAGGAAGCAGAATCGCAATCG |
RTG3-R | ACAGGTGAGTCGTAAGCCATAG | |
PRB1 | PRB1-F | AAGGTGGAGGAGAAGAAGATG |
PRB1-R | CAGTGGACACGAGAGGAG | |
APG14 | APG14-F | GGTCCGTCTCGCTATATTGG |
APG14-R | ATGAGGTCCTGTGACTGTTG | |
DAL80 | DAL80-F | GTGCTTAGTGATTCGTTGA |
DAL80-R | ACCGTGTTCATCTCTTCT | |
MIG2 | MIG2-F | CAGGCAGTAAATGGAGGTA |
MIG2-R | AATTGGCATAGGAGAAGTTG | |
MOH1 | MOH1-F | TGAGCTTCATCACCTACGGTTGTA |
MOH1-R | GTCGCCAGTCAACATTCTTCGT | |
ECM37 | ECM37-F | GCCACCTGATTCCTCGTCAA |
ECM37-R | GCGAATGCCCTGTCTTACTCA | |
YLR257W | YLR257W-F | CTCCTTCTCTGCGTGCTTCCA |
YLR257W-R | CGTCGTCTGCCTCACCTGTAAT | |
GDH2 | GDH2-F | CCATGCTCGTGTGAGGAACT |
GDH2-R | CGAACACCCAGAGAAGTCATACC | |
MEP3 | MEP3-F | GGTGGTTGGTTGACGCATA |
MEP3-R | TCGGCTTCCTCAGTGACT | |
URK1 | URK1-F | AGGTATAGGTGGTGCTTCAGGTT |
URK1-R | GGCTCTGGCTCTGTCCTCTG | |
GLN1 | GLN1-F | CGCCAAGGAAGGTTACGGTTA |
GLN1-R | CTTCGTCATGTCAGCATTGTCAA | |
DIP5 | DIP5-F | TCATCCACCTCTACATCACCTTCA |
DIP5-R | GAACCACCAATGGCGATCATAGAA | |
PUT4 | PUT4-F | CTGAGATTCCGCAAGGCTATT |
PUT4-R | CCCGTTGGTAATGGTGATGAT | |
YHM1 | YHM1-F | TGCCTCATACCGATAAGAAACAAT |
YHM1-R | TTGGTGTGGTTGGACATCAG | |
GZF3 | GZF3-F | GCGAAGAAGAAGGGCATATTGG |
GZF3-R | GCTCCTGCTTGCGATTCAC | |
VID30 | VID30-F | TGACGACGACGACGATGA |
VID30-R | TTCCTTCTTCTCCACCTTCCT | |
DAL5 | DAL5-F | GGAAGACAGAGGAACAATTCACAA |
DAL5-R | GGTTCTCCAGCCTTTAATAGCATA | |
DAL4 | DAL4-F | GGAGACCACTTACACCAGAG |
DAL4-R | CCGTCATATCAGCACCACAT | |
DAL7 | DAL7-F | GGAGTACAGGTTGAAGGATGACAT |
DAL7-R | GAAGATGGAAGCACTAATCGGTTC | |
MEP2 | MEP2-F | AAGTGGACTACAGTTGGT |
MEP2-R | AACAGCAAGGTTACATCC | |
CPS1 | CPS1-F | TTATTATCATCGGCACCTTCTTC |
CPS1-R | GGACTTAATGGTTCAATCTTCTCA | |
NIT1 | NIT1-F | TGAGAAGGAGATCAAGGAATC |
NIT1-R | TTCGGCAAGATACTTAGCATA | |
GAT1 | GAT1-F | GACGATGACGATGATGATGAC |
GAT1-R | ACCAGAAGCATTATGTGAAGC | |
DSD1 | DSD1-F | GAATGGTATGTAGCAAGGGTCTCT |
DSD1-R | ACAAGCGTGTTGAGGAAGGA | |
ALD4 | ALD4-F | GAAGGTAGAGAGGACGATGT |
ALD4-R | ACCGTTCCAAGACCCATTA | |
PUT2 | PUT2-F | AGAGTCAGCGGATGGTACTTGGAA |
PUT2-R | TAATTTGAAAGGGCAGCGGTTTGT | |
UGA1 | UGA1-F | GCCGTACTATCGTTCAAGAGA |
UGA1-R | GCCAAGCAATGGTCATCCT | |
ARG4 | ARG4-F | CGTCTCTTCCGTATGATTA |
ARG4-R | CCAATTCTGTCTCCGTTA | |
ACT1 | ACT1-F | GGTTGCTGCTTTGGTTATTG |
ACT1-R | CCTTGGTGTCTTGGTCTAC |
