Figure 7: POFUT2 is indispensable for ADAMTS9 secretion whereas B3GLCT is required for optimal secretion.
From: Impaired ADAMTS9 secretion: A potential mechanism for eye defects in Peters Plus Syndrome
(a) HEK293T cells were co-transfected with siRNA targeting POFUT2, or B3GLCT, or a non-targeting control (Neg. Ctrl.), and hADAMTS9-N-L2 or human IgG heavy chain. Media and lysates were analyzed by western blotting with anti-myc (hADAMTS9-N-L2) or anti-human IgG. ADAMTS-N-L2 in medium migrates more rapidly compared to the cell lysate because the N-terminal propeptide (~25 kDa) is excised by furin at the cell-surface. The western blot images are cropped from gels which were provided for review as Supplementary information (Supplementary Fig. S7). (b) Quantitation of data from the western blot is shown relative to the control siRNA (Neg. Ctrl.). Data are mean+/− standard deviation (n = 3). Significance was determined using a 2-tailed Student’s t test (*p < 0.005, #p < 0.001).
