Figure 4
From: High Fructose Diet inducing diabetes rapidly impacts olfactory epithelium and behavior in mice
Eight weeks of HFruD reduces the sensitivity and changes the kinetics of odorant induced responses in MOR23 neurons.
(a) Dose–response relationships of MOR23 cells in response to Lyral: inward currents induced by Lyral at different concentrations (indicated by the numbers in mol/l) under voltage clamp with one example of a control cell (a1) and a HFruD cell (a2). (b) The dose–response curves of the peak currents from control (n = 11 cells from 6 mice, b1) and HFruD (n = 12 cells from 6 mice, b2). The black symbols and thick black line correspond to the average (±SEM) of the control or HFruD neurons. (c) Quantification of the dose-response characteristics: bar graphs representing the average maximum amplitude (c1), the average K1/2 the concentration at which half of the maximum response was reached (c2) and the average Hill coefficient (c3). (d) Quantification of the kinetics characteristics of control and HFruD MOR23 neurons in response to 10−5 M Lyral: analysis of the maximum amplitude (d1, control: n = 16, HFruD: n = 10), the rise-time (d2, control: n = 17, HfruD: n = 10), the total current elicited (d3, control: n = 16, HFruD: n = 10) and the time at 50% (d4, control: n = 17, HFruD: n = 10). All recordings performed in perforated patch and at a membrane potential of −67mV. Data represented as mean ± SEM; *p < 0.05 after Student’s t test.
