Figure 1: Significantly elevated expression of FAIM2 in SCLC compared to NSCLC cell lines. | Scientific Reports

Figure 1: Significantly elevated expression of FAIM2 in SCLC compared to NSCLC cell lines.

From: FAIM2, as a novel diagnostic maker and a potential therapeutic target for small-cell lung cancer and atypical carcinoid

Figure 1

(A) Microarray data from a total of 187 lung cancer cell lines (133 NSCLC and 54 SCLC) available in the CCLE database were analyzed for Fas-apoptosis pathway genes including FADD, FAS, CASP8, and FAIM2. While pro-apoptotic genes, FADD, FAS, and CASP8 were significantly underexpressed in SCLC cells compared to NSCLC cells (p < 0.0001), FAIM2 expression was significantly overexpressed in SCLC cells compared to NSCLC cells (p = 0.0002). (FADD: 1.8 fold increase of expression in SCLC, FAS: 4.9 fold increase of expression in SCLC, CASP8: 3.0 fold increase of expression in SCLC: FAIM2: 0.8 fold decrease of expression in SCLC- Fold change was calculated from the average values). NSCLC: black dot. SCLC: gray dot. RMA: Robust Multi-array Average. (B) Four SCLC and seven NSCLC cell lines were analyzed by real-time quantitative RT-PCR for FAIM2 mRNA expression. Two Taqman assays detecting exons 2–3 (colored in gray) and exons 5–6 (colored in black) in the FAIM2 transcript were used for FAIM2 expression analysis. FAIM2 expression was significantly higher in SCLC than those of NSCLC cell lines (p < 0.0001). (C) The result from Taqman assays was confirmed by semi-quantitative RT-PCR. FAIM2 expression was clearly detected in four SCLC cell lines while no clear band was detected in NSCLC cell lines. GAPDH was used as a housekeeping gene control.

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