Figure 7

Freezing tolerance of all mutant lines (see Fig. 1) compared to the corresponding wild types, of non-acclimated and cold acclimated plants.

Mutant lines in Col-0 (framed in yellow) and Ler (framed in orange) are sorted according to their position in the flavonoid biosynthetic pathway. The bars indicate the differences in leaf freezing tolerance between wild type and mutant expressed as LT₅₀ (temperature at which 50% electrolyte leakage occurred). Positive values indicate improved freezing tolerance, negative values impaired freezing tolerance compared to the respective wild type. Data are means from two to four independent plant cultures, each with four biological replicates per line and condition. Significant differences, evaluated by Welch’s unpaired t-test, are marked by asterisks: *p < 0.05, **p < 0.01, ***p < 0.001.