Figure 3

SAM chronic administration reversed the cognitive deficits of McGill-Thy1-APP mice.

(A–D) Deficits in the Morris water maze. (A) Latency to locate the hidden platform during the learning phase of the test. Animals were given 4 trials per day for 5 days, with the first 2 trials of day 1 involving a visible platform. Mean latency is calculated as area under the curve (AUC, right panel). (B–D) Memory recall was assessed 24 h after completion of the learning phase and time spent in the target quadrant (B), time to first reach the platform (C) and number of crosses over the annulus that contained the platform (D) were analysed. (E) Mice were tested for spontaneous activity in open-field. (F,G) Deficits in Novel Object Recognition test. (F) No preference for either of the 2 objects was recorded during the training phase of the task, when 2 identical objects were presented. (G) During the probe test, carried 24 h after training, WT mice showed a clear preference for the new object, while the vehicle-treated Tg mice failed to discriminate between the familiar and novel object. Treatment with SAM20 reduced this deficit. The 0.5 discrimination ratio, equivalent to chance is indicated by a dashed line. Data are expressed as mean ± SEM and analyzed with One-Way ANOVA, followed by Bonferroni post hoc test; *p < 0.05 vs WT Veh; ^#p < 0.05 vs Tg Veh.