Figure 3
Bioinformatics of phosphorylation sites.
(a) Intensity map showing the relative abundance of 12 amino acid residues flanking the class one phosphorylation sites. Different colors in the intensity map represent log10 of the ratio of amino acid frequency occurring within 13 residues from the pS/pT/pY residue versus within 13 amino acids from all S/T/Y residues. (b) In vivo phosphorylation sites, determined by Motif-X. The height of each amino acid represents the frequency of this residue occurring at the position of pS/pT. (c) Bar chart showing kinases targeting the class one phosphorylation sites, determined with NetworKIN. Only the results with both a NetworKIN score ≥1.0 and a String score ≥0.6 were considered. (d) Predicted secondary structures and accessibility of phosphorylation sites. Probabilities of different secondary structures (alpha-helix, beta-strand and coil) and accessibility of pS/pT/pY were compared with those of all S/T/Y residues in all phosphoproteins identified in this study.
