Figure 6: The in vitro neuronal stretch injury model.
From: Stretch Injury of Human Induced Pluripotent Stem Cell Derived Neurons in a 96 Well Format
(A) The injury device consists of a stage positioned above an array of Teflon-coated, aluminum posts and driven vertically by an electromagnetic voice coil. (B) The silicone-bottomed plate consists of a commercially-distributed plate top covalently bonded to a sheet of silicone. Since no sandwich construction or air tight gaskets are employed, the standard geometry of the 96 well plate is preserved. (C) Schematic depiction of the injury process. The plate and post array are shown in cross section. Initially, the plate is positioned so that the silicone membrane touches the posts. To induce injury, the plate is lowered, stretching the membrane over the rims of the posts. Posts can be omitted from the post array to create unstretched, control wells.
