Figure 3: Reduction in Rad51 expression resulted in defective chromosome segregation and spindle assembly in MI oocytes. | Scientific Reports

Figure 3: Reduction in Rad51 expression resulted in defective chromosome segregation and spindle assembly in MI oocytes.

From: The role of Rad51 in safeguarding mitochondrial activity during the meiotic cell cycle in mammalian oocytes

Figure 3

(A) Immunofluorescence staining for the spindle and chromosomes in MI oocytes after injection with GFP- (a,c–f) or Rad51- (b,g–j) dsRNA in GV oocytes that were then allowed to mature in vitro. The oocytes were fixed in 4% paraformaldehyde and then stained with an antibody against Pericentrin (red) and α-Tubulin (green). DNA was counterstained with DAPI (blue). Lower panel shows the magnified region of the spindle-chromosome complex in another MI oocyte after GFP RNAi (c–f) and Rad51 RNAi (g–j). The scale bars indicate 20 μm. (B) Proportion of GFP or Rad51-dsRNA injected MI oocytes with defects of spindle assembly and chromosome configuration. (C) RT-PCR analysis of the expression of MTOC-related genes, including Aurka, Aurkc, Bora, Cep192, Pcnt, Plk1 and Tacc3, in Rad51-silenced MI oocytes. H1foo was used as an internal control.

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