Figure 6: Despite the accumulation of mitochondria by 3-MA, these mitochondria were malfunctional. | Scientific Reports

Figure 6: Despite the accumulation of mitochondria by 3-MA, these mitochondria were malfunctional.

From: The role of Rad51 in safeguarding mitochondrial activity during the meiotic cell cycle in mammalian oocytes

Figure 6

(A) Immunofluorescence staining for spindles and chromosomes after GFP (a,b,e,f) or Rad51 RNAi (c,d,g,h) and cultured without (a–d) or with 10 mM 3-MA (e–h). Oocytes were immunostained for spindles (α-Tubulin; green), mitochondria (MitoTracker; red) and chromosomes (DAPI; blue). The scale bars indicate 20 μm. (B) Effect of mitophagy inhibition on ΔΨm after Rad51 silencing. ΔΨm was measured using JC-1 staining. The ratio of RITC to FITC intensity was calculated. The scale bars indicate 20 μm. (C) Quantitative analysis of ΔΨm associated with Rad51 disruption and mitophagy inhibition is summarized. The data are presented as the mean ± SEM. Different letters (a~c) indicate significant differences at p < 0.05. (D) Effects of mitophagy inhibition on ATP levels in Rad51 down-regulated oocytes. ATP levels were measured as described in the Materials and Methods. The data are presented as the mean ± SEM. Different letters (a~c) indicate significant differences at p < 0.05.

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