Figure 4 | Scientific Reports

Figure 4

From: Tumor-targeted delivery of a C-terminally truncated FADD (N-FADD) significantly suppresses the B16F10 melanoma via enhancing apoptosis

Figure 4

FADD and N-FADD delivered by S. typhimurium strain VNP20009 expressed in vitro and in vivo under the control of NirB promoter.

(A) pQE30-NirB-FADD and pQE30-NirB-N-FADD plasmids were constructed and transformed into VNP (VNP-pN-FADD and VNP-pN-N-FADD). VNP-pN-FADD and VNP-pN-N-FADD were grown for 72 h under anaerobic or aerobic conditions. Bacterial lysates were subjected to immunoblotting assays using anti-FADD (N term) antibodies (B). (C) The relative tissue distribution of indicated stable strains was detected by colony formation assay. *p < 0.001, tumor compared with the other tissues. Data are expressed as mean ± SD of five animals. (D) Detection of the expression of FADD or N-FADD in tumor tissues of the mice bearing melanoma by western blotting. β-actin was served as loading control. (E) Statistical analysis the results of western blot by Image J; **P < 0.01, compared with PBS group. One representative of three independent experiments is displayed.

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