Figure 5: Electrophysiological features of LQTS3/BrS iPSC-derived cardiomyocytes following SCN3B knockdown, as determined by patch-clamp analyses.
From: Embryonic type Na+ channel β-subunit, SCN3B masks the disease phenotype of Brugada syndrome
(A) Quantitative RT-PCR analyses of SCN3B in iPSC-derived cardiomyocytes transfected with either scrambled siRNA (n = 3) or siRNA for SCN3B (n = 3). (B) Average current–voltage relationship for peak current in control and LQTS3/BrS iPSC-derived cardiomyocytes transfected with scrambled siRNA or siRNA for SCN3B (n = 10 for all groups). (C,E) Average voltage dependency of activation (C) and inactivation (E) in control and LQTS3/BrS iPSC-derived cardiomyocytes transfected with scrambled siRNA or siRNA for SCN3B. (D,F) V½ of activation (D) and inactivation (F) in control and LQTS3/BrS iPSC-derived cardiomyocytes transfected with scrambled siRNA (n = 9 and 13, respectively) or siRNA for SCN3B (n = 10 and 11, respectively). Where appropriate, data are given as the mean ± SEM. *P < 0.05 compared with control.
