Table 1 The linearity range, calibration curve, correlation coefficient (R2), limit of detection (LOD), and recovery of FAs.
FAs | Linearity (n = 3) | LOD (μM) | QC (%) | Recovery (%, n = 3) | ||||
|---|---|---|---|---|---|---|---|---|
FA (μM) | Calibration curve | R2 | R1 | R2 | R3 | |||
C16:0 | 5.0~412.5 | Y = 1.184(±0.062)X + 0.256(±0.067) | 0.996 | 0.5 | <16 | 78.8 | 94.7 | 95.7 |
C16:1 | 1.3~103.1 | Y = 0.427(±0.234)X + 0.054(±0.018) | 0.998 | 0.1 | <17 | 95.5 | 110.0 | 99.6 |
C18:0 | 7.5~625.0 | Y = 1.176(±0.061)X−0.147(±0.204) | 0.991 | 2.2 | <17 | 92.8 | 79.6 | 93.9 |
C18:1 | 7.5~625.0 | Y = 0.877(±0.081)X + 0.177(±0.236) | 0.996 | 1.5 | <17 | 92.7 | 95.2 | 97.9 |
C18:2 | 7.5~625.0 | Y = 1.059(±0.041)X−0.139(±0.083) | 0.996 | 1.9 | <18 | 93.4 | 92.2 | 97.7 |
C18:3 | 0.6~51.6 | Y = 1.405(±0.114)X + 0.011(±0.024) | 0.995 | 0.3 | <18 | 107.8 | 105.5 | 91.1 |
C20:4 | 2.5~206.3 | Y = 1.047(±0.077)X−0.205(±0.269) | 0.998 | 0.1 | <18 | 96.6 | 86.1 | 91.9 |
C22:6 | 1.3~103.1 | Y = 0.925(±0.087)X−0.031(±0.122) | 0.993 | 0.3 | <16 | 85.9 | 96.8 | 90.6 |
