Figure 4: PRL1 also plays a role in maintaining spermatogenesis.

(A) Testis sections from 3 months or 2 weeks old male mice were histologically examined by H&E staining and immunohistochemical staining for Vimentin, PLZF and Kit. (B–E) Relative seminiferous tubule diameters (B), number of Vimentin positive cell per seminiferous tubule (C), number of c-Kit positive cell per tubule (D) and number of PLZF positive cell per tubule (E) in testis sections from all five genotypes were quantificated. For each mouse, at least 20 tubules were counted. *p < 0.05, **p < 0.01 (Student’s t test). Data are representative of at least three independent experiments (mean and SD). Scale bar = 50 μm.