Figure 6: Total PRL1 and PRL2 negatively correlated with PTEN.
From: Role of phosphatase of regenerating liver 1 (PRL1) in spermatogenesis
(A) Alterations of key signaling pathway molecules in testis from different genotypes by Western blot. (B) Upper panel: germ cells isolated from testis of male mice with different genotypes were analyzed for PTEN, pAkt, Akt, Actin, PRL1 and PRL2 by Western blot. Lower panel: relative level of either PTEN/Actin, pAkt/Akt or total PRL1 and PRL2/Actin in isolated germ cells from different genotypes. (C) PTEN immunohistochemistry analysis in seminiferous tubules of testis with different genotypes. (D) Quantification of PTEN IHC staining by ImageJ IHC Image Analysis Toolbox. *p < 0.05 (Student’s t test) compared to wild-type; #p < 0.05 (Student’s t test) compared to PRL2−/− and PRL1−/−/PRL2+/−. Scale bar = 50 μm.
