Figure 3: Time course of TLR4 inhibition in the presence of B. quintana LPS. | Scientific Reports

Figure 3: Time course of TLR4 inhibition in the presence of B. quintana LPS.

From: Bartonella quintana lipopolysaccharide (LPS): structure and characteristics of a potent TLR4 antagonist for in-vitro and in-vivo applications

Figure 3

Human PBMCs were isolated from healthy subjects, using a standard protocol. PBMCs were pre-incubated with 100 ng/ml or 1000 ng/ml B. quintana LPS for 2 h. Thereafter 10 ng/ml purified E. coli LPS was added as indicated in the graph. Cytokines were determined after 24 h, 48 h or 72 h of culture by standard ELISA for IL-1β (A), TNF-α (B), IL-6 (C) and IL-8 (D). PBMCs of 6 healthy donors were examined. *P < 0.001, two-sided Mann-Whitney U test.

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