Figure 5: Kinetics of the B. quintana LPS to block the TLR4. | Scientific Reports

Figure 5: Kinetics of the B. quintana LPS to block the TLR4.

From: Bartonella quintana lipopolysaccharide (LPS): structure and characteristics of a potent TLR4 antagonist for in-vitro and in-vivo applications

Figure 5

Human PBMCs were isolated from healthy subjects, using a standard protocol. (A) PBMCs were pre-incubated with 100 ng/ml or 1000 ng/ml B. quintana LPS for different times before E. coli LPS was added to the culture medium. After 1 hour, 45, 30 and 15 minutes PBMC were exposed to 10 ng/ml E. coli LPS for 24 h. (B) B. quintana LPS was added together with E. coli LPS, thereafter the PBMCs were cultured for additional 24 h, 48 h or 72 h. C, B. quintana LPS was added in a range before (−2 h) and after (+2 h) the cells were exposed to 10 ng/ml E. coli LPS. IL-6 was determined by using ELISA. PBMCs of 4 subjects were used in this experiment. *P < 0.001, two-sided Mann-Whitney U test.

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