Figure 2
Minocycline prevents glomerular inflammasome activation.
(a,b) In db/db mice treated with minocycline, but not in those treated with CIX, expression of Nlrp3 as well as the cleaved form of IL1β (cl-IL1β, b) are reduced in renal cortex extracts. (c,d) Immunohistochemical detection of Nlrp3 and cleaved caspase-1 demonstrates that minocycline, but not CIX, reduces markers of inflammasome activation within diseased glomeruli of db/db mice. (e,f) In glomeruli cleaved caspase-1 (cl-Casp1, red, e,f) co-localizes with the podocyte marker synaptopodin (Syn, green, e) and the endothelial marker CD34 (green, f). Minocycline, but not CIX application, reduces co-localization for both cell-types. Mean values ± SEM (a,b,d).; size bar (c,e): 20 μm; *P < 0.05 (a,b,d: ANOVA). Number of mice in each group is shown in parentheses in (a); (a,b) Representative cropped images without further modification are shown; exemplary uncropped images are provided in Supplementary Fig. 6.
