Figure 2: IFT-B complex is destabilized in IFT81 mutants.

(A) RNA levels of IFT81 in control and patient chondrocytes. β-Actin serves as loading control. (B) Protein levels of several components of IFT-B complex (IFT81, IFT74, IFT88 and IFT52), acetyl-tubulin and kinesin motor component (KIF3A). GADPH serves as a loading control. (C–H) Bar graphs showing statistical analyses (t-Test) for the replicates of each studied protein between control and R98-443. (I) Cartoon of the IFT-B complex transporting αβ-tubulin through a cilia microtubule.