Figure 4 | Scientific Reports

Figure 4

From: Aguamiel concentrate from Agave salmiana and its extracted saponins attenuated obesity and hepatic steatosis and increased Akkermansia muciniphila in C57BL6 mice

Figure 4

Aguamiel concentrate and its saponins prevented adipose tissue hypertrophy, hyperleptinemia and increased UCP-1 expression.

(A) H&E staining of epididymal adipose tissue (200x magnification). (B) Immunohistochemical determination of F4/80-positive cells in epididymal adipose tissue (400x magnification). (C) H&E staining of subcutaneous adipose tissue (200x magnification). (D) Epididymal adipocyte size distribution. (E) Plasma leptin concentration and (F) correlation between epididymal fat and plasma leptin. (G) Brown subcutaneous adipose tissue H&E staining (200x magnification). (H) Epididymal and (I) subcutaneous UCP-1 immunohistochemical staining (400x magnification). Subcutaneous adipose tissue gene expression of (J) UCP1 and (K) TBX1. The statistical analysis was performed with one-way ANOVA followed by the Bonferroni post hoc test. Mean values with different letters where a > b > c at the same adipocyte size are significantly different (at least P < 0.05). C: control; HF: High-fat; HFAC: High-fat with aguamiel concentrate; HFL: High-fat with low saponin dose; HFH: High-fat with high saponin dose.

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