Figure 2 | Scientific Reports

Figure 2

From: Pomegranate extract decreases oxidative stress and alleviates mitochondrial impairment by activating AMPK-Nrf2 in hypothalamic paraventricular nucleus of spontaneously hypertensive rats

Figure 2

Effects of pomegranate extract supplementation on superoxide anion and chronic neuronal activation in the hypothalamic paraventricular nucleus of control and spontaneously hypertensive rats.

Spontaneously hypertensive rats and controls were administered either saline or pomegranate extract (150 mg/kg/day) for eight weeks, sacrificed and frozen sections were prepared from the paraventricular nucleus. (A) A representative immunofluorescence image showing superoxide production as detected with dihydroethidium staining (DHE, red fluorescence). (B) A representative immunofluorescence image showing Fra-Like expression (green fluorescence). Statistical analysis of dihydroethidium (C) and Fra-Like (D) positive neurons in the paraventricular nucleus of hypertensive and control rats, with or without pomegranate extract treatment. Values are the mean ± SEM from 10 animals. *p < 0.05, **p < 0.01. 3V, third ventricle; SHR, spontaneously hypertensive rats; PE, pomegranate extract, PVN, paraventricular nucleus.

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