Figure 8 | Scientific Reports

Figure 8

From: Pomegranate extract decreases oxidative stress and alleviates mitochondrial impairment by activating AMPK-Nrf2 in hypothalamic paraventricular nucleus of spontaneously hypertensive rats

Figure 8

Effects of pomegranate extract supplementation on mitophagy and the expression of AMPK-Nrf2 pathway components in the hypothalamic paraventricular nucleus of control and hypertensive rats.

(A) Mitophagy (red fluorescence) was measured using the fluorescent probe Lysotracker, which was detected with confocal microscopy. (B) Statistical analysis of the number of the mitophagy-positive neurons. (C,D,E,F) The protein expression levels of AMPK, p-AMPK, Nrf2 and hemeoxygenase were analyzed by Western blotting (B, Western blotting image; C,D,E, statistical analysis). Values are the mean ± SEM from 8 animals. **p < 0.01, ***p < 0.001. SHR, spontaneously hypertensive rats; PE, pomegranate extract; PVN, paraventricular nucleus; Nrf2, nuclear factor-erythroid 2 p45-related factor 2; HO-1, hemeoxygenase.

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