Figure 1

(a) Morphology of phiAxp-2 plaques. Phages were plated in Luria–Bertani agar and overlain with a liquid culture of A. xylosoxidans A22732. The plates were incubated at 37 °C. Clear, well-defined phiAxp-2 plaques were observed and photographed after 18 h. (b) Phage morphology. Phage was stained with 2% phosphotungstic acid and visualized at 120,000-fold magnification with transmission electron microscopy. Scale bars represent 100 nm. (c) One-step growth curve of the bacteriophage. The phage was grown in an exponential-phase culture of A. xylosoxidans strain A22732. Shown are the pfu per infected cell in the cultures at different time points. Each data point is the mean of three experiments.