Figure 3: Western blot quantification of the 22 kDa glycoprotein and 14-3-3 protein.
From: Comparative Secretome Analysis Reveals Perturbation of Host Secretion Pathways by a Hypovirus
The protein was detected with specific polyclonal antibodies. An amount of 50 μg of protein per sample from three independent extractionswas loaded and separated on 12% PAGE. After transfer to a PVDF membrane, the blot was detected by 22 kDa glycoprotein-specific antibody or 14-3-3 protein-specific antibody. Lanes 1–3 represent samples from independent preparations (A). Semi-quantification of the blots indicated that the 22 kDa glycoprotein was expressed basically at the same level in the fungal strains, EP155 and EP713, while 14-3-3 protein was significantly down-regulated in EP713 (−2.5 fold) as compared with that of EP155, consisting well with the quantification results of iTRAQ analysis (B).
